Characterisation of Populus alba L. by isozymes
Abstract
A characterisation of Populus alba L by isozymes has been carried out. Eight isoenzymatic systems were analysed: ADH, MDH, GOT, LAP, PGM, PGI, SKDH, and IDH; all of them showing polymorphic loci. A total of 18 loci, 12 of them polymorphic, and 33 alleles were identified. Several half-sib and full-sib families from controlled crosses have been analysed in order to establish the genetic interpretation of the loci. Mendelian segregation was tested in controlled crosses families by Chi-square test (goodness fit) for five loci: Adh-2, Got-1, Got-2, Pgi-2 and Mdh-3. Segregation distortion was observed in Got-1, Got-2 and Pgi-2 in several families; in this cases pool of families was anlysed. Genetic control was determined in half-sib families for: Adh-2, Got-1, Got-2, Mdh-1, Mdh-3, Pgm-2 and Skdh; significant differences from the mendelian segregation were not found in this case. A putative asignation of loci and alleles was infered in LAP and IDH. This study identify a total of 18 loci, as an useful tool for studying diversity in Populus alba.Downloads
© CSIC. Manuscripts published in both the printed and online versions of this Journal are the property of Consejo Superior de Investigaciones Científicas, and quoting this source is a requirement for any partial or full reproduction.
All contents of this electronic edition, except where otherwise noted, are distributed under a “Creative Commons Attribution 4.0 International” (CC BY 4.0) License. You may read here the basic information and the legal text of the license. The indication of the CC BY 4.0 License must be expressly stated in this way when necessary.
Self-archiving in repositories, personal webpages or similar, of any version other than the published by the Editor, is not allowed.