Influencia de los métodos de conservación en las actividades enzimáticas de suelos forestales mediterráneos

  • M. Andrés Abellan Instituto de Investigación en Energías Renovables (IER). Sección de Medio Ambiente.
  • C. Wic Baena Instituto de Investigación en Energías Renovables (IER). Sección de Medio Ambiente.
  • F.A. García Morote Instituto de Investigación en Energías Renovables (IER). Sección de Medio Ambiente.
  • M.I. Picazo Cordoba Instituto de Investigación en Energías Renovables (IER). Sección de Medio Ambiente.
  • D. Candel Pérez Departamento de Ciencia y Tecnología Agroforestal y Genética.
  • M.E. Lucas-Borja Instituto de Investigación en Energías Renovables (IER). Sección de Medio Ambiente.Departa
Palabras clave: enzima del suelo, técnica de almacenamiento del suelo, método de conservación, análisis enzimáticos.

Resumen

El método de conservación del suelo utilizado puede alterar la actividad enzimática, siendo decisivas para los resultados, las condiciones de conservación previas a los análisis. Para los estudios es preferible realizar los análisis en muestras frescas y recién cogidas. Sin embargo, esto no es siempre posible por razones prácticas ya que las muestras se tienen que recoger en un corto periodo del año o porque los análisis los debe hacer poca gente y siempre en un determinado tiempo. En este contexto, se necesita un método de conservación, siendo el mantenimiento en el frigorífico a 4°C el método más usado, aunque existen otros métodos alternativos. El objetivo de este trabajo es el de evaluar el efecto de dos métodos alternativos de conservación (congelado a –20°C y secado a temperatura ambiente) sobre las actividades enzimáticas (deshidrogenasa, fosfatasa, β-glucosidasa y ureasa), en comparación con el mantenido en el frigorífico a 4°C. Las muestras se obtuvieron de dos zonas forestales diferentes (pinar y encinar) y en dos épocas distintas (primavera y verano de 2009). Los resultados mostraron diferencias en las actividades enzimáticas cuando se usó el congelado o secado al aire de las muestras de suelo en comparación con el mantenimiento en el frigorífico. De forma general, los métodos de conservación alternativos mostraron una menor actividad enzimática en las muestras de suelo analizadas. Sin embargo, estos cambios dependen de la época del año y la zona de muestreo.

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Publicado
2011-12-04
Cómo citar
Andrés Abellan, M., Wic Baena, C., García Morote, F., Picazo Cordoba, M., Candel Pérez, D., & Lucas-Borja, M. (2011). Influencia de los métodos de conservación en las actividades enzimáticas de suelos forestales mediterráneos. Forest Systems, 20(3), 379-388. https://doi.org/10.5424/fs/20112003-11081
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