Plant regeneration of Populus alba "Siberia extremeña" from ament

  • M. A. Bueno INIA
  • R. Astorga INIA
  • J. A. Manzanera Universidad Politécnica de Madrid, Madrid
Keywords: Tissue culture, micropropagation, Populus alba, thidiazurone, adventious buds


Populus alba "Siberia Extremeña", a clone of great rusticity and high interest for poplar culture, is difficult to propagate by conventional cutting methods. Plant regeneration from ament callus has been achieved in Murashige-Skoog (MS) medium supplemented with 2 mg/l of 2,4 dichlorofenoxyacetic acid (2,4-D) y 1 mg/l of kinetin. Optimal adventitious bud production arised from callus treated with 1 mg/l of thidiazuron and 0,1 mg/l of α-naphtaleneacetic acid (NAA). Induced buds were subcultured in modified MS mediun plus 0,1 mg/l of 6-benzyladenine (BA) and 0,002 mg/l of NAA. Shoots were rooted in Woody Plant Medium (WPM) plus 0,2 mg/l of indole-3-butyric acid (IBA), reaching a 95 p. 100 rooting. Acclimation of plantlets to glasshouse and nursery conditions rendered a 96 p. 100 survival tax.


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How to Cite
Bueno, M. A., Astorga, R., & Manzanera, J. A. (1992). Plant regeneration of Populus alba "Siberia extremeña" from ament. Forest Systems, 1(2), 163-171.
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