Molecular characterization of Spanish Prunus avium plus trees
Abstract
Aim of the study: The Breeding Program of wild cherry (Prunus avium) developed by Lourizán Forest Research Center (NW Spain), aims for the creation of the Main Breeding Population, that is formed by a large number of plus trees and for obtaining an Elite Population generated from controlled crosses of a number of plus trees selected by, at least, one trait of economic importance. The aim of this study was to genotype 131 accessions of Prunus avium plus trees, included in the breeding program.
Area of study: The Prunus avium plus trees are located in the North, Northwest and Central Spain.
Material and Methods: The Prunus avium plus trees were genotyped with nine microsatellites. Several genetic parameters were calculated. Genetic data were analyzed with STRUCTURE and the genetic distance between the plus trees were calculated.
Main results: A total of 122 multilocus genotypes were detected. Several accessions with the same genotype were identified, which could be due to clonality or to labelling errors. The nine microsatellites are useful for identifying individuals because the combined probability of identity was low (PI = 5.19X10-9). Bayesian methods detected two genetic clusters in the sampled plus trees.
Research highlights: The unique genotypes identified in this work are suitable for being included in the elite breeding population for economic traits.
Keywords: Prunus avium; breeding program; microsatellite; genetic distance.
Downloads
References
Arnaud-Haond S, Belkhir K, 2007. GENCLONE: A computer program to analyse genotypic data, test for clonality and describe spatial clonal organization. Mol. Ecol. Notes 7: 15-17. http://dx.doi.org/10.1111/j.1471-8286.2006.01522.x
Chapuis M-P, Lecoq M, Michalakis Y, Loiseau A, Sword GA, Piry S, et al., 2008. Do outbreaks affect genetic population structure? A worldwide survey in Locusta migratoria, a pest plagued by microsatellite null alleles. Mol. Ecol. 17: 3640-3653. http://dx.doi.org/10.1111/j.1365-294X.2008.03869.x
Clarke JB, Tobutt KR, 2003. Development and characterization of polymorphic microsatellites from Prunus avium "Napoleon." Mol. Ecol. Notes 3: 578-580. http://dx.doi.org/10.1046/j.1471-8286.2003.00517.x
De Cuyper B, Sonneveld T, Tobutt KR, 2005. Determining self-incompatibility genotypes in Belgian wild cherries. Mol. Ecol. 14: 945-955. http://dx.doi.org/10.1111/j.1365-294X.2005.02460.x
De Rogatis A, Ferrazzini D, Ducci F, Guerri S, Carnevale S, Belletti P, 2013. Genetic variation in Italian wild cherry (Prunus avium L.) as characterized by nSSR markers. Forestry 86: 391-400. http://dx.doi.org/10.1093/forestry/cpt009
Dorken ME, Eckert CG, 2001. Severely reduced sexual reproduction in northern populations of a clonal plant, Decodon verticillatus (Lythraceae). J. Ecol. 89: 339-350. http://dx.doi.org/10.1046/j.1365-2745.2001.00558.x
Ellstrand NC, Roose ML, 1987. Patterns of genotypic diversity in clonal plant species. Am. J. Bot. 74: 123-131. http://dx.doi.org/10.2307/2444338
Evanno G, Regnaut S, Goudet J, 2005. Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study. Mol. Ecol. 14: 2611-2620. http://dx.doi.org/10.1111/j.1365-294X.2005.02553.x
Felsenstein J, 1989. PHYLIP - Phylogeny Inference Package (Version 3.2). Cladistics 5: 164-166.
Guarino C, Santoro S, Simone LD, Cipriani G, 2009. Prunus avium: nuclear DNA study in wild populations and sweet cherry cultivars. Genome 52: 320-337. http://dx.doi.org/10.1139/G09-007
Hardy OJ, Vekemans X, 2002. SPAGeDI: A versatile computer program to analyse spatial genetic structure at the individual or population levels. Mol. Ecol. Notes 2: 618-620. http://dx.doi.org/10.1046/j.1471-8286.2002.00305.x
Hubisz MJ, Falush D, Stephens M, Pritchard JK, 2009. Inferring weak population structure with the assistance of sample group information. Mol. Ecol. Resour. 9: 1322- 1332. http://dx.doi.org/10.1111/j.1755-0998.2009.02591.x
Pritchard JK, Stephens M, Donnelly P, 2000. Inference of population structure using multilocus genotype data. Genetics 155: 945-959.
Serres-Giardi L, Dufour J, Russell K, Buret C, Laurens F, Santi F, 2010. Natural triploids of wild cherry. Can. J. For. Res. 40: 1951-1961. http://dx.doi.org/10.1139/X10-100
Tanceva Crmaric O, Stambuk S, Satovic Z, Kajba D, 2011. Genotypic diversity of wild cherry (Prunus avium L.) In the part of its natural distribution in croatia. Sumar. List 135: 543-555.
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P, 2004. MICRO-CHECKER: Software for identifying and correcting genotyping errors in microsatellite data. Mol. Ecol. Notes 4: 535-538. http://dx.doi.org/10.1111/j.1471-8286.2004.00684.x
Vaughan SP, Russell K, 2004. Characterization of novel microsatellites and development of multiplex PCR for large-scale population studies in wild cherry, Prunus avium. Mol. Ecol. Notes 4: 429-431. http://dx.doi.org/10.1111/j.1471-8286.2004.00673.x
Von Schelhorn M, 1947. Über eine triploide Vogelkirsche. Der Züchter 17-18: 232-235.
© CSIC. Manuscripts published in both the printed and online versions of this Journal are the property of Consejo Superior de Investigaciones Científicas, and quoting this source is a requirement for any partial or full reproduction.
All contents of this electronic edition, except where otherwise noted, are distributed under a “Creative Commons Attribution 4.0 International” (CC BY 4.0) License. You may read here the basic information and the legal text of the license. The indication of the CC BY 4.0 License must be expressly stated in this way when necessary.
Self-archiving in repositories, personal webpages or similar, of any version other than the published by the Editor, is not allowed.
