Transferibilidad cruzada de microsatélites a cinco especies de Araucariaceae: una herramienta útil para estudios de genética de poblaciones en Araucaria araucana
Resumen
La transferencia de cebadores que amplifican loci microsatélites desde otras especies es una práctica habitual para obtener marcadores adecuados para estudios de genética poblacional. Los cebadores diseñados en una especie (fuente) son utilizados para amplificar loci homólogos en especies relacionadas (blanco). Se espera que especies cercanas filogenéticamente compartan un mayor número de marcadores y la distancia genética entre especies podría ser un parámetro útil para predecir el éxito de la transferencia entre diferentes grupos taxonómicos. Se analizó la transferencia de veintidós primers, desarrollados para Araucaria angustifolia (Bertol.) Kuntze, a cinco especies blanco de la familia Araucariaceae. Los resultados se resumieron en vectores de presencia y ausencia de bandas y fueron comparados a través del índice de similitud de Jaccard. Se estimaron las distancias genéticas de a pares entre las especies analizadas utilizando las secuencias de ocho genes, y se las relacionó con el índice de transferencia utilizando correlaciones de Pearson. El éxito de la transferencia varió entre 31.8 y 77.3%, encontrándose estos valores entre los más altos reportados en plantas. El mayor índice de transferencia se verificó entre las especies sudamericanas. La transferencia fue confirmada secuenciando siete fragmentos amplificados en Araucaria araucana (Molina) K. Koch, y utilizando los cinco mejores para estimar parámetros de diversidad genética en una población natural de esta conífera andina.Descargas
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Barreneche T., Casasoli M., Russell K., Akkak A., Meddour H., Plomion C., Villani F., Kremer A., 2004. Comparative mapping between Quercus and Castanea using simple-sequence repeats (SSRs). Theoretical and Applied Genetics 108, 558-566. http://dx.doi.org/10.1007/s00122-003-1462-2 PMid:14564395
Bassam B.J., Caetano-Anollés G., Gresshoff P.M., 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry 196, 80-83. http://dx.doi.org/10.1016/0003-2697(91)90120-I
Bekessy S.A., Allnutt T.R., Premoli A.C., Lara A., Ennos R.A., Burgman M.A., Cortes M., Newton A.C., 2002. Genetic variation in the vulnerable and endemic Monkey Puzzle tree (Araucaria araucana (Molina) K. Koch), detected using RAPDs. Heredity 88, 243-249. http://dx.doi.org/10.1038/sj.hdy.6800033 PMid:11920130
Brondani R.P.V., Brondani C., Tarchini R., Grattapaglia D., 1998. Development, characterization and mapping of microsatellite markers in Eucalyptus grandis and E. urophylla. TAG Theoretical and Applied Genetics 97, 816-827. http://dx.doi.org/10.1007/s001220050961
Brown R.C., Tsalavouta M., Terzoglou V., Magoulas A., Mcandrew B.J., 2005. Additional microsatellites for Sparus aurata and cross-species amplification within the Sparidae family. Molecular Ecology Notes 5, 605-607. http://dx.doi.org/10.1111/j.1471-8286.2005.01007.x
Csaikl U.M., Bastian H., Brettschneider R., Gauch S., Meir A., Schauerte M., Scholz F., Sperisen C., Vornam B., Ziegenhagen B., 1998. Comparative analysis of different DNA extraction protocols: a fast, universal maxi-preparation of high quality plant DNA for genetic evaluation and phylogenetic studies. Plant Molecular Biology Reporter 16, 69-86. http://dx.doi.org/10.1023/A:1007428009556
Dowling T.E., Moritz C., Palmer J.D., Rieseberg L.H., 1996. Nucleic acids III: analysis of fragments and restriction sites. In: Molecular systematics (Hillis D.M., Moritz C., Mable B.K., eds). Sinauer, Sundeland, Massachusetts, USA. pp. 249-320.
Durand J., Bodenes C., Chancerel E., Frigerio J.-M., Vendramin G., Sebastiani F., Buonamici A., Gailing O., Koelewijn H.-P., Villani F., Mattioni C., Cherubini M., Goicoechea P., Herran A., Ikaran Z., Cabane C., Ueno S., Alberto F., Dumoulin P.-Y., Guichoux E., De Daruvar A., Kremer A., Plomion C., 2010. A fast and cost-effective approach to develop and map ESTSSR markers: oak as a case study. BMC Genomics 11, 570-583. http://dx.doi.org/10.1186/1471-2164-11-570 PMid:20950475 PMCid:3091719
Farjon A., Page C.N. (eds), 1999. Conifers: status survey and conservation action plan. Gland, Switzerland and Cambridge, UK, IUCN.
Feng S., Li W., Huang H., Wang J., Wu Y., 2009. Development, characterization and cross-species/genera transferability of EST-SSR markers for rubber tree (Hevea brasiliensis). Molecular Breeding 23, 85-97. http://dx.doi.org/10.1007/s11032-008-9216-0
Fisher P.J., Richardson T.E., Gardner R.C., 1998. Characteristics of single- and multi-copy microsatellites from Pinus radiata. TAG Theoretical and Applied Genetics 96, 969-979. http://dx.doi.org/10.1007/s001220050828
Fitzsimmons N.N., Moritz C., Moore S.S., 1995. Conservation and dynamics of microsatellite loci over 300 million years of marine turtle evolution. Molecular Biology and Evolution 12, 432-440. PMid:7739385
Gallo L., Izquierdo F., Sanguinetti L.J., Pinna A., Siffredi G., Ayesa J., López C., Pelliza A., Strizler N., Gonzales Peñalba M., Maresca L., Chauchard L., 2004. Los recursos genéticos silvícolas de Araucaria araucana en Argentina. Desafíos de la ordenación de los recursos genéticos silvícolas para contribuir a la subsistencia: ejemplos de Argentina y Brazil. [on line]. Available in http://www2.bioversityinternational.org/publications/1140/. [21 Jan, 2011].
Guidugli M.C., Guerrieri Accoronii K.A., Mestrineri M.A., Betioli Conteli E.P., Martínez C.A., Alzate-Marin A.L., 2010. Genetic characterization of 12 heterologous microsatellite markers for the giant tropical tree Cariniana legalis (Lecythidaceae). Genetics and Molecular Biology 33, 131-135. http://dx.doi.org/10.1590/S1415-47572010000100022 PMid:21637616 PMCid:3036079
Hall T.A., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41, 95-98.
Heesacker A., Kishore V.K., Gao W., Tang S., Kolkman J.M., Gingle A., Matvienko M., Kozik A., Michelmore R.M., Lai Z., Rieseberg L.H., Knapp S.J., 2008. SSRs and INDELs mined from the sunflower EST database: abundance, polymorphisms, and cross-taxa utility. Theoretical and Applied Genetics 117, 1021-1029. http://dx.doi.org/10.1007/s00122-008-0841-0 PMid:18633591
Hendre P.S., Phanindranath R., Annapurna V., Lalremruata A., Aggarwal R.K., 2008. Development of new genomic microsatellite markers from robusta coffee (Coffea canephora Pierre ex A. Froehner) showing broad cross-species transferability and utility in genetic studies. BMC Plant Biology 8, 51-70. http://dx.doi.org/10.1186/1471-2229-8-51 PMid:18447947 PMCid:2396172
Hill R.S., Scriven L.J., 1995. The angiosperm-dominated woody vegetation of Antarctica: a review. Review of Palaeobotany and Palynology 86, 175-198. http://dx.doi.org/10.1016/0034-6667(94)00149-E
Holmen J., Vøllestad L.A., Jakobsen K.S., Primmer C.R., 2005. Cross-species amplification of zebrafish and central stoneroller microsatellite loci in six other cyprinids. Journal of Fish Biology 66, 851-859. http://dx.doi.org/10.1111/j.0022-1112.2005.00637.x
Isagi Y., Suhandono S., 1997. PCR primers amplifying microsatellite loci of Quercus myrsinifolia Blume and their conservation between oak species. Molecular Ecology 6, 897-899. http://dx.doi.org/10.1111/j.1365-294X.1997.tb00147.x PMid:9301079
Jamieson A., Taylor S.C.S., 1997. Comparisons of three probability formulae for parentage exclusion. Animal Genetics 28, 397-400. http://dx.doi.org/10.1111/j.1365-2052.1997.00186.x PMid:9616104
Kayser M., Ritter H., Bercovitch F., Mrug M., Roewer L., Nürnberg P., 1996. Identification of highly polymorphic microsatellites in the rhesus macaque Macaca mulatta by cross-species amplification. Molecular Ecology 5, 157-159. http://dx.doi.org/10.1111/j.1365-294X.1996.tb00302.x PMid:9147691
Kijas J.M.H., Fowler J.C.S., Thomas M.R., 1995. An evaluation of sequence tagged microsatellite site markers for genetic analysis within Citrus and related species. Genome 38, 349-355. http://dx.doi.org/10.1139/g95-045 PMid:7774802
Kumar S., Tamura K., Nei M., 1993. MEGA: molecular evolutionary genetics analysis, version 1.01. University Park, TPS University.
Kupper C., Burke T., Szekely T., Dawson D., 2008. Enhanced cross-species utility of conserved microsatellite markers in shorebirds. BMC Genomics 9, 502-522. http://dx.doi.org/10.1186/1471-2164-9-502 PMid:18950482 PMCid:2588463
Liewlaksaneeyanawin C., Ritland C.E., Elkassaby Y.A., Ritland K., 2004. Single-copy, species-transferable microsatellite markers developed from loblolly pine ESTs. Theoretical and Applied Genetics 109, 361-369. http://dx.doi.org/10.1007/s00122-004-1635-7 PMid:15045175
Lin G., Chang A., Yap H., Yue G., 2008. Characterization and cross-species amplification of microsatellites from the endangered Hawksbill turtle (Eretmochelys imbricate). Conservation Genetics 9, 1071-1073. http://dx.doi.org/10.1007/s10592-007-9459-z
Liu N., Zhu Y., Wie Z., Chen J., Qb W., Jian S., Zhou D., Shi J., Yang Y., Zhong Y., 2009. Phylogenetic relationships and divergence times of the family Araucariaceae based on the DNA sequences of eight genes. Chinese Science Bulletin 54, 2648-2655. http://dx.doi.org/10.1007/s11434-009-0373-2
Marchelli P., Baier C., Mengel C., Ziegenhagen B., Gallo L., 2010. Biogeographic history of the threatened species Araucaria araucana (Molina) K. Koch and implications for conservation: a case study with organelle DNA markers. Conservation Genetics 11, 951-963. http://dx.doi.org/10.1007/s10592-009-9938-5
Mottura M.C., Finkeldey R., Verga A.R., Gailing O., 2005. Development and characterization of microsatellite markers for Prosopis chilensis and Prosopis flexuosa and cross-species amplification. Molecular Ecology Notes 5, 487-489. http://dx.doi.org/10.1111/j.1471-8286.2005.00965.x
Nei M., 1971. Interspecific gene differences and evolutionary time estimated from electrophoretic data on protein identity. The American Naturalist 105, 385-398. http://dx.doi.org/10.1086/282732
Patreze C., Tsai S., 2010. Intrapopulational genetic diversity of Araucaria angustifolia (Bertol.) Kuntze is different when assessed on the basis of chloroplast or nuclear markers. Plant Systematics and Evolution 284, 111-122. http://dx.doi.org/10.1007/s00606-009-0238-9
Peakall R., Ebert D., Scott L.J., Meagher P.F., Offord C.A., 2003. Comparative genetic study confirms exceptionally low genetic variation in the ancient and endangered relictual conifer, Wollemia nobilis (Araucariaceae). Molecular Ecology 12, 2331-2343. http://dx.doi.org/10.1046/j.1365-294X.2003.01926.x PMid:12919472
Peakall R., Smouse P.E., 2006. GenAlEx 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6, 288-295. http://dx.doi.org/10.1111/j.1471-8286.2005.01155.x
Primmer C.R., Painter J.N., Koskinen M.T., Palo J.U., Merilä J., 2005. Factors affecting avian crossspecies microsatellite amplification. Journal of Avian Biology 36, 348-360. http://dx.doi.org/10.1111/j.0908-8857.2005.03465.x
R-Development-Core-Team, 2008. R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.
Rallo P., Tenzer I., Gessler C., Baldoni L., Dorado G., Martín A., 2003. Transferability of olive microsatellite loci across the genus Olea. Theoretical and Applied Genetics 107, 940-946. http://dx.doi.org/10.1007/s00122-003-1332-y PMid:12827252
Rico C., Rico I., Hewitt G., 1996. 470 million years of conservation of microsatellite loci among fish species. Proceedings of the Biological Sciences 263, 549-557. http://dx.doi.org/10.1098/rspb.1996.0083 PMid:8677258
Robertson A., Hollingsworth P.M., Kettle C.J., Ennos R.A., Gardner M.F., 2004. Characterization of nuclear microsatellites in New Caledonian Araucaria species. Molecular Ecology Notes 4, 62-63. http://dx.doi.org/10.1046/j.1471-8286.2003.00569.x
Rohlf F.J., 2001. NTSYS: numerical taxonomy and multivariate analysis system version 2.1. Setauket. E. Software. PMCid:64842
Rossetto M., Harriss F.C.L., Mclauchlan A., Henry R.J., Baverstock P.R., Lee L.S., 2000. Interspecific amplification of tea tree (Melaleuca alternifolia-Myrtaceae) microsatellite loci-potential implications for conservation studies. Australian Journal of Botany 48, 367-373. http://dx.doi.org/10.1071/BT98084
Rossetto M., 2001. Sourcing of SSR markers from related plant species. In: Plant genotyping: the DNA fingerprinting of plants (Henry R.J., ed). CAB International, Wallingford, UK. pp. 211-224. http://dx.doi.org/10.1079/9780851995151.0211
Ruiz E., González F., Torres-Díaz C., Fuentes G., Mardones M., Stuessy T., Samuel R., Becerra J., Silva M., 2007. Genetic diversity and differentiation within and among Chilean populations of Araucaria araucana (Araucariaceae) based on allozyme variability. Taxon 56, 1221-1219. http://dx.doi.org/10.2307/25065913
Salgueiro F., Caron H., De Souza M.I.F., Kremer A., Margis R., 2005. Characterization of nuclear microsatellite loci in South American Araucariaceae species. Molecular Ecology Notes 5, 256-258. http://dx.doi.org/10.1111/j.1471-8286.2005.00899.x
Scott L.J., Shepherd M., Henry R.J., 2003. Characterization of highly conserved microsatellite loci in Araucaria cunninghamii and related species. Plant Systematics and Evolution 236, 115-123. http://dx.doi.org/10.1007/s00606-002-0217-x
Scott L.J., 2004. Implications of evolutionary history and population structure for the analysis of quantitative trait loci in the ancient conifer Araucaria cunninghamii. PhD thesis. Lismore, NSW, Southern Cross University. pp. 155.
Scott L., Shepherd M., Nikles D., Henry R., 2005. Low efficiency of pseudotestcross mapping design was consistent with limited genetic diversity and low hetero- zygosity in hoop pine (Araucaria cunninghamii, Araucariaceae). Tree Genetics and Genomes 1, 124-134. http://dx.doi.org/10.1007/s11295-005-0022-0
Schmeda-Hirschmann G., Astudillo L., Rodríguez J., Theoduloz C., Yáñez T., 2005. Gastroprotective effect of the Mapuche crude drug Araucaria araucana resin and its main constituents. Journal of Ethnopharmacology 101, 271-276. http://dx.doi.org/10.1016/j.jep.2005.04.027 PMid:15985351
Schmidt A.B., Ciampi A.Y., Guerra M.P., Nodari R.O., 2007. Isolation and characterization of microsatellite markers for Araucaria angustifolia (Araucariaceae). Molecular Ecology Notes 7, 340-342. http://dx.doi.org/10.1111/j.1471-8286.2006.01602.x
Stefenon V., Gailing O., Finkeldey R., 2004. Genetic structure of Araucaria angustifolia (Araucariaceae) populations in Brazil: implications for the in situ conservation of genetic resources. Plant Biology 9, 516-525. http://dx.doi.org/10.1055/s-2007-964974 PMid:17401807
Stefenon V.M., 2007. Rotas de migração da Araucária. Ciência Hoje. 39, 59-61.
Steinkellner H., Lexer C., Turetschek E., Glössl J., 1997. Conservation of (GA)n microsatellite loci between Quercus species. Molecular Ecology 6, 1189-1194. http://dx.doi.org/10.1046/j.1365-294X.1997.00288.x
Tamura K., Dudley J., Nei M., Kumar S., 2007. Mega4: molecular evolutionary genetics analysis (MEGA) Software Version 4.0. Molecular Biology and Evolution 24, 1596-1599. http://dx.doi.org/10.1093/molbev/msm092 PMid:17488738
Van Oosterhout C., Hutchinson W.F., Wills D.P.M., Shipley P., 2004. MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes 4, 535-538. http://dx.doi.org/10.1111/j.1471-8286.2004.00684.x
Ven W.T.G., Mcnicol R J., 1996. Microsatellites as DNA markers in Sitka spruce. Theoretical and Applied Genetics 93, 613-617. http://dx.doi.org/10.1007/BF00417956
Zhang D.X., Hewitt G.M., 2003. Nuclear DNA analyses in genetic studies of populations: practice, problems and prospects. Molecular Ecology 12, 563-584. http://dx.doi.org/10.1046/j.1365-294X.2003.01773.x PMid:12675814
Zhang Y., Sledge M.K., Bouton J.H., 2007. Genome mapping of white clover (Trifolium repens L.) and comparative analysis within the Trifolieae using cross-species SSR markers. Theoretical and Applied Genetics 114, 1367-1378. http://dx.doi.org/10.1007/s00122-007-0523-3 PMid:17356868 PMCid:1950584
Zucchi M.I., Brondani R.P.V., Pinheiro J.B., Brondani C., Vencovsky R., 2002. Transferability of microsatellite markers from Eucalyptus spp. to Eugenia dysenterica (Myrtaceae family). Molecular Ecology Notes 2, 512-513. http://dx.doi.org/10.1046/j.1471-8286.2002.00297.x
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