Cambios estacionales en la actividad fisiológica del regenerado bajo un gradiente natural de luz en una masa natural de Pinus pinea.
Resumen
Durante 2007 y 2008 se midió la evolución estacional de la fluorescencia a la clorofila, de los parámetros de intercambio gaseoso y del estado hídrico, junto con variables ambientales, en el regenerado de cuatro clases de edad de pino piñonero (Pinus pinea L.) bajo un gradiente natural de luz en una masa regular de pino piñonero. Las plantas de un año se adaptaron peor a las condiciones de sequía extrema del verano (principalmente en 2008), mostrando valores menores de Fv/Fm al mediodía y al amanecer, de tasa fotosintética al mediodía, de Phi-PSII, de potenciales hídricos (Phi-pd y Phi-min) y una mayor mortalidad (90%). Las plantas de mayor edad (clase 4, 51-200 cm de altura), aunque no experimentaron los valores de Phi-min menores en julio 2008, no fueron capaces de mantener una fotosíntesis positiva al mediodía, incluso mostrando los valores menores. Sin embargo, se recuperaron rápidamente al suavizarse las condiciones climáticas en otoño. A finales de otoño se obtuvieron valores bajos de Fv/Fm, especialmente en las plantas de un año, indicando que la máxima eficiencia fotoquímica del PSII fue sensible a las temperaturas bajo cero registradas en ambos otoños. Por tanto, la regeneración del pino piñonero adapta su fisiología a los cambios estacionales de temperatura y disponibilidad hídrica, mostrando un comportamiento fotosintético diferente según la clase de edad. Sin embargo, la fisiología de las plantas no se vió afectada por el gradiente natural de luz encontrado en la masa estudiada, probablemente debido a la baja variabilidad registrada en GSF y a la baja densidad de la masa. Se sugiere que esa densidad es insuficiente para asegurar la supervivencia de las plantas de un año, pero suficiente para el regenerado de más edad.Descargas
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Annaz D., Karmiloff-Smith A., Johnson M.H., Thomas M.S.C., 2009. A cross-syndrome study of the development of holistic face recognition in children with autism, Down syndrome, and Williams syndrome. J Exp Child Psychol 102, 456-486. http://dx.doi.org/10.1016/j.jecp.2008.11.005 PMid:19193384
Aussenac G., 2000. Interactions between forest stands and microclimate: ecophysiological aspects and consequences for silviculture. Ann For Sci 57, 287-301. http://dx.doi.org/10.1051/forest:2000119
Barbeito I., Pardos M., Calama R., Cañellas I., 2008. Effect of stand structure on Stone pine (Pinus pinea L.) regeneration dynamics. Forestry. doi: 10.1093/forestry/cpn037. http://dx.doi.org/10.1093/forestry/cpn037
Bond B.J., 2000. Age-related changes in photosynthesis of oody plants. Trends Pl Sci Rev 5, 349-353. http://dx.doi.org/10.1016/S1360-1385(00)01691-5
Calama R., Montero G., 2007. Cone and seed production from stone pine (Pinus pinea L.) stands in Central Range (Spain). Eur J For Res 126, 23-35 . http://dx.doi.org/10.1007/s10342-005-0100-8
Castro J., Zamora R., Hódar J.A., Gómez J.M., 2005. Alleviation of summer drought boosts establishment success of Pinus sylvestris in a Mediterranean mountain: an experimental approach. Plant Ecol 181, 191-202. http://dx.doi.org/10.1007/s11258-005-6626-5
Chaves M.M., Pereira J.S., Maroco J., Rodrigues M.L., Ricardo C.P.P., Osorio M.L., Carvalho I., Faria T., Pnheiro C., 2002. How plants cope with water stress in the field. Photosynthesis and growth. Ann Bot-London 89, 907-916. http://dx.doi.org/10.1093/aob/mcf105
Chaves A.R.M., Ten-Caten A., H.A., Ribeiro A., Damatta F.M., 2008, Seasonal changes in photoprotective mechanisms of leaves from shaded and unshaded field-grown coffe (Coffea Arabica L.) trees. Trees 22, 351-361. http://dx.doi.org/10.1007/s00468-007-0190-7
Climent J., Costa E Silva F., Chambel M.R., Pardos M., Almeida M.H., 2009. Freezing injury in primary and secondary needles of Mediterranean pine species of contrasting ecological niches. Ann For Sci 66(4), 407. http://dx.doi.org/10.1051/forest/2009016
Cordeiro Y.E.M., Pinheiro H.A., Dos Santos Filho B.G., Corrêa S.S., E Silva J.R.R., Dias-Filho M.B., 2009. Physiological and morphological responses of young mahogany (Swietenia macrophyllaa King) plants to drought. For Ecol Managem 258, 1449-1455.
Dawson T.E., Ehleringer J.R., 1993. Gender-specific physiology, carbon isotope discrimination and habitat distribution in boxelder. Acer negundo. Ecology 74, 798-815. http://dx.doi.org/10.2307/1940807
Dawson T.E., 1996. Determining water use by trees and forests from isotopic, energy balance and transpiration analysis: the roles of tree size and hydraulic lift. Tree Physiol 16, 263-272. http://dx.doi.org/10.1093/treephys/16.1-2.263 PMid:14871771
Delaney H.D., Maxwell S.E., 1981. On using analysis of covariance in repeated measures designs. Multivariate Behavioral Res 16, 105-123. http://dx.doi.org/10.1207/s15327906mbr1601_6
Demmig-Adams B., Adams W.W., Witer K., Meyer A., Schreiber U., Pereira J.S., Krüger A, Czygan F.C., Lagne O.L., 1989. Photochemical efficiency of photosystem II, photon yield of O2 evolution, photosynthesis capacity and carotenoid composition during the midday depression of net CO2 uptake in Arbutus under grown in Portugal. Planta 177, 377-387. http://dx.doi.org/10.1007/BF00403596
Donovan L.A., Ehleringer J.R., 1991. Ecophysiological differences among juvenile and reproductive plants of several woody species. Oecologia 86, 594-597. http://dx.doi.org/10.1007/BF00318327
Epron D., Dreyer E., Bréda N., 1992. Photosynthesis of oak trees [Quercus petraea (Matt) Liebl] during drought under field conditions: diurnal courses of net CO2 assimilation and photochemical efficiency of photosystem II. Plant Cell Environ 15, 809-820. http://dx.doi.org/10.1111/j.1365-3040.1992.tb02148.x
Filella I., Llusià J., Piñol J., Peñuelas J., 1998 Leaf gas exchange and fluorescence of Phyllirea latifolia, Pistacia lentiscus and Quercus ilex saplings in severe drought and high temperature conditions. Environ Exp Bot 39, 213-220. http://dx.doi.org/10.1016/S0098-8472(97)00045-2
Flexas J., Medrano H., 2002. Drought-inhibition of photosynthesis in C3 plants: stomatal and non-stomatal limitation revisited. Ann Bot 89, 183-189. http://dx.doi.org/10.1093/aob/mcf027
Fotelli M.N., Radoglou K.M., Constantinidou H.I.A., 2000. Water stress responses of seedlings of four Mediterranean oak species. Tree Physiol 20, 1065-1075. http://dx.doi.org/10.1093/treephys/20.16.1065 PMid:11269958
García-Plazaola J.I., Faria T., Abadía J., Abadía A., Chaves M.M., Pereira J.S., 1997. Seasonal changes in xantophyll composition and photosynthesis of Cork oak (Quercus suber L.) leaves under mediterraean climate. J Exp Bot 48, 1667-1674.
Hernández-Santana V., Martínez-Vilalta J., Martínez-Fernández J., Williams M., 2009. Evaluating the effect of drier and warmer conditions on water use by Quercus pyrenaica. For Ecol Managem 258, 1719-1730.
Irvine J., Perks P., Magnani F., Grace J. 1998. The response of Pinus sylvestris to drought: stomatal control of transpiration and hydraulic conductance. Tree Physiol 18, 393-402. http://dx.doi.org/10.1093/treephys/18.6.393 PMid:12651364
Keenan T., García R., Friend A.A., Zaehle S., Gracia C., Sabaté S., 2009. Improved understanding of drought control seasonal variation in Mediterranean forest canopy CO2 and water fluxes through combined in situ measurements and ecosystem modelling. Biogeosciences 6, 1423-1444. http://dx.doi.org/10.5194/bg-6-1423-2009
Kolb T.E., Stone J.E., 2000. Differences in leaf gas exchange and water relations among species and tree sizes in an Arizona pine-oak forest. Tree Physiol 20, 1-12. http://dx.doi.org/10.1093/treephys/20.1.1 PMid:12651521
Kozlowski T.T., Pallardy S.G., 2007. Physiology of woody plants. Academic Press, San Diego, California. 411 pp.
Latham R.E., 1992. Co-occurring tree species change rank in seedling performance with resources varied experimentally. Ecology 73(6), 2129-2144. http://dx.doi.org/10.2307/1941461
Llusiá J., Peñuelas J., 2000. Seasonal patterns of terpene content and emission from seven Mediterranean woody species in field conditions.
Manetas Y., Petropoulou Y., Stamatakis K., Nikolopoulos D., Levizou E., Psaras G., Karabourniotis G., 1997. Beneficial effects of enhaced UV-B radiation under field conditions: improvement of needle water relations and survival capacity of Pinus pinea L. seedlings during the dry Mediterranean summer. Plant Ecol 128, 100-108. http://dx.doi.org/10.1023/A:1009706806427
Martínez-Ferri E., Balaguer L., Valladares F., Chico J.M., Manrique E., 2000. Energy dissipation in drought-avoiding and drought-tolerant tree species at midday during the Mediterranean summer. Tree Physiol 20, 131-138 http://dx.doi.org/10.1093/treephys/20.2.131 PMid:12651481
Masetti C., Mencuccini M., 1991. Régéneration naturelle du pin pignon (Pinus pinea L.) dans la Pineta Granducale di Alberese (Parco Naturale della Maremma, Toscana, Italie). Ecol Mediterr 17, 103-118 .
Matzner S.L., Rice K.J., Richards J.H., 2003. Patterns of stomatal conductance among blue oak (Quercus douglassi) size classes and populations: implications for seedling establishment. Tree Physiol 23, 777-784. http://dx.doi.org/10.1093/treephys/23.11.777 PMid:12839731
Mayseyk K., Grünzweig J.M., Rotenberg E., Yakir D., 2008. Respiration acclimation contributes to high carbon-use efficiency in a seasonally dry pine forest. Glob. Change Biol 14, 1553-1567. http://dx.doi.org/10.1111/j.1365-2486.2008.01604.x
Mayseyk K., Lin T., Rotenberg E., Grünzweig J.M., Schwartz A., Yakir D., 2008. Physiology-phenology interactions in a productive semi-arid pine forest. New Phytol 178, 603-616. http://dx.doi.org/10.1111/j.1469-8137.2008.02391.x PMid:18331428
Mendoza I., Zamora R., Castro J., 2009. A seeding experiment for testing tree-community recruitment under variable environments: implications for forest regeneration and conservation in mediterranean habitats. Biolog Conservat 142, 1491-1499. http://dx.doi.org/10.1016/j.biocon.2009.02.018
Montero G., Calama R., Ruiz-Peinado R., 2008. Selvicultura de Pinus pinea L. In: Compendio de selvicultura aplicada en España (Serrada R., Montero G., Reque J.A., eds). INIA, MEC, Madrid. pp. 432-470.
Moser E.B., Saxton A.M., Pezeshki S.R., 1990. Repeated measures analysis of variance: application to tree research, Can J For Res 20, 524-535. http://dx.doi.org/10.1139/x90-069
Ogaya R., Peñuelas J., 2003a. Comparative seasonal gas exchange and chlorophyll fluorescence of two dominant woody species in a holm oak forest. Flora 198, 132-141. http://dx.doi.org/10.1078/0367-2530-00085
Ogaya R., Peñuelas J., 2003b. Comparative field study of Quercus ilex and Phillyrea latifolia: photosynthetic response to experimental drought conditions. Environ Exp Bot 50, 137-148. http://dx.doi.org/10.1016/S0098-8472(03)00019-4
Oliveira G., Peñuelas J., 2000. Comparative photochemical and phenomorphological responses to winter stress of an evergreen (Quercus ilex) and a semi-deciduous (Cistus albidus) Mediterranean woody species. Acta Oecol 21, 97-107. http://dx.doi.org/10.1016/S1146-609X(00)00121-1
Oliveira G., Peñuelas J., 2001. Allocation of absorbed light energy into photochemistry and dissipation in a semi-deciduous and an evergreen Mediterranean woody species during winter. Aust J Plant Physiol 28, 471-480.
Oliveira G., Peñuelas J., 2004. The effect of winter cold stress on photosynthesis and photochemical efficiency of PSII of two Mediterranean woody species- Cistus albidus and Quercus ilex. Plant Ecol 175, 179-191. http://dx.doi.org/10.1007/s11258-005-4876-x
Öquist G., Gardeström P., Huner N.P.A., 2001 Metabolic changes during cold acclimation and subsequent freezing and thawing. In: Conifer cold hardiness (Bigras F.J., Colombo S.J., eds). Kluwer Academic Publishers, Dordrecht,The Netherlands. pp. 137-163.
Panek J.A., Goldstein A.H., 2001. Response of stomatal conductance to drought in ponderosa pine: implications for carbon and ozone uptake. Tree Physiol 21, 337-344. http://dx.doi.org/10.1093/treephys/21.5.337 PMid:11262925
Pardos M., Ruiz Del Castillo J., Cañellas I., Montero G., 2005. Ecophysiology of natural regeneration of forest stands in Spain. Invest Agrar: Sist Recur For 14, 434-445.
Peñuelas J., Llusiá J., 2002. Linking photorespiration, monoterpenes and plant termotolerance. New Phytol 155, 227-237. http://dx.doi.org/10.1046/j.1469-8137.2002.00457.x
Peñuelas J., Prieto P., Beier C. et al. 2007. Response of plant species richness and primary productivity in shrublands along a north-south gradient in Europe to seven years of experimental warming and drought. Reductions in primary productivity in the heat and drought year of 2003. Glob Change Biol 13, 2563-2581. http://dx.doi.org/10.1111/j.1365-2486.2007.01464.x
Petropoulou Y., Kyparissis A., Nikolopoulos D., Manetas Y., 1995. Enhanced UV-B radiation alleviates the adverse effects of summer drought in two Mediterranean pines under field conditions. Physiol Plantar 94, 37-44. http://dx.doi.org/10.1111/j.1399-3054.1995.tb00781.x
Poyatos R., Llorens P., Piñol J., Rubio C., 2008. Response of Scots pine (Pinus sylvestris L.) and pubescent oak (Quercus pubescens Willd.) to soil and atmospheric water deficits under Mediterranean mountain climate. Ann For Sci 65, 306. http://dx.doi.org/10.1051/forest:2008003
Powers M.D., Pregitzer K.S., Palik B.J., Webster C.R., 2009. Water relations of pine seedlings in contrasting overstory environments. For Ecol Managem 258, 1442-1448.
Prieto P., Peñuelas J., Llusià J., Asensio D., Estiarte M., 2009. Effects of long-term experimental night-time warming and drought on photosynthesis, Fv/Fm and stomatal conductance in the dominant species of a Mediterranean shrubland. Acta Physiol Plant 31, 729-739. http://dx.doi.org/10.1007/s11738-009-0285-4
Rodríguez-Calcerrada J., Pardos J.A., Gil L., Aranda I., 2007. Summer field performance of Quercus petraea (Matt.) Liebl and Quercus pyrenaica Willd seedlings, planted in three sites with contrasting canopy cover. New Forests 33, 67-80. http://dx.doi.org/10.1007/s11056-006-9014-7
Resco De Dios V., Fishcher C., Colinas C., 2007. Climate change effects on mediterranean forests and preventive measurements. New Forests 33, 29-40. http://dx.doi.org/10.1007/s11056-006-9011-x
SAS Institute, 1988. SAS/STAT users guide. Release 6.03 edition. Cary, USA.
Savé R., Castell C., Terradas J., 1999. Gas exchange and water relations. In: Ecology of Mediterranean evergreen oak forests (Rodá F., Retana J., Gracia C.A., Bellot J., eds). Springer-Verlag, Berlin. pp. 135-147. http://dx.doi.org/10.1007/978-3-642-58618-7_10
Tenhunen J.D., Serra A.S., Harley P.C., Dougherty R.L., Reynolds J.F., 1990. Factors influencing carbon fixation and water-use by Mediterranean sclerophyll shrubs during summer drought. Oecologia 82, 381-393. http://dx.doi.org/10.1007/BF00317487
Tenhunen J.D., Lange O.L., Braun M., 1981. Midday stomatal closure in mediterranean type sclerophylls under simulated habitat conditions in an environmental chamber. II. Effect of the complex of leaf temperature and air humidity on gas exchange of Arbutus unedo and Quercus ilex. Oecologia 50, 5-11. http://dx.doi.org/10.1007/BF00378788
Thomas S.C., 2010. Photosynthetic capacity peaks at intermediate size in temperate deciduos trees. Tree Physiol 30, 555-573. http://dx.doi.org/10.1093/treephys/tpq005 PMid:20335160
Thomas M.S.C., Annaz D., Ansari D., Serif G., Jarrold C., Karmiloff-Smith A., 2009. Using developmental trajectories to understand developmental disorders. J Speech, Language, and Hearing Res 52, 336-358. http://dx.doi.org/10.1044/1092-4388(2009/07-0144)
Valladares F., Pearcy R.W., 1997. Interactions between water stress, sun-shade acclimation, heat tolerance and photoinhibition in the sclerophyll Heteromeles arbutifolia. Plant Cell Environ 20, 25-36. http://dx.doi.org/10.1046/j.1365-3040.1997.d01-8.x
Valladares F., Dobarro I., Sánchez-Gómez D., Pearcy R.W., 2005. Photoinhibition and drought in mediterranean woody saplings: scaling effects and interactions in sun and shade phenotypes. J Exp Bot 56, 483-494. http://dx.doi.org/10.1093/jxb/eri037 PMid:15569705
Werner C., Correia O., Beyschlag W., 1999. Two different strategies of Mediterranean macchia plants to avoid photoinhibitory damage by excessive radiation levels during summer drought. Acta Oecol 20, 15-23. http://dx.doi.org/10.1016/S1146-609X(99)80011-3
Zunzunegui M., Fernández-Baco L., Díazbarradas M.C., García.Novo F., 1999. Seasonal changes in photochemical efficiency in leaves of Halimium halimifolium, a Mediterranean semideciduous shrub. Photosynthetica 31, 17-31. http://dx.doi.org/10.1023/A:1007058611140
Zweifel R., Zimmermann L., Newbery D.M., 2005. Modelling tree water deficit from microclimate: an approach to quantifying drought stress. Tree Physiol 25, 147-156 http://dx.doi.org/10.1093/treephys/25.2.147 PMid:15574396
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