Formamide deionized accelerates the somatic embryogenesis of Cunninghamia lanceolata

  • Shichan He Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037
  • Zhaodong Hao Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037
  • Dandan Wang Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037
  • Yulin Guo Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037
  • Hua Wu Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037
  • Asif Ali Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037
  • Renhua Zheng Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China, Fujian Academy of Forestry, Fuzhou, 350012
  • Xuenyan Zheng National Germplasm Bank of Chinese fir at Fujian Yangkou Forest Farm, Shunchang 353211, China
  • Jinhui Chen Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037
  • Jisen Shi Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037
DOI: https://doi.org/10.5424/fs/2021303-18068

Abstract

Aim of the study: To improve the efficiency of the somatic embryogenesis (SE) in Cunninghamia lanceolata.

Area of the study: The study was conducted at Nanjing Forestry University (Nanjing, China).

Material and methods: Immature cones of C. lanceolata, genotype 01A1 which was planted in Yangkou State-owned Forest Farm (Fujian, China), were used to induced callus. These calli were used to induce SE, concentration gradients of 0 g/L, 0.01134 g/L, 0.1134 g/L, 1.1134 g/L and 11.34 g/L of FD was added, to explore the optimal concentration for promoting SE of C. lanceolata.

Main results: Low concentration of FD promoted the maturation of somatic embryos, while high concentration of FD lead to browning of embryogenic callus. The seedling rate and rooting number of seedlings induced by different concentrations of FD were significantly different.

Research highlights: This study may aid in the rapid maturation of C. lanceolata somatic embryos and is useful for accelerated C. lanceolata breeding.

Keywords: C. lanceolata; Formamide Deionized; Somatic embryogenesis; Seedling rate.

Abbreviations used: FD (Formamide Deionized), FD0 (the concentration of 0 g/L FD), FD0.01134 (the concentration of 0.01134 g/L FD), FD0.1134 (the concentration of 0.1134 g/L FD), FD1.134 (the concentration of 1.134 g/L FD), FD11.34 (the concentration of 11.34 g/L FD).

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Published
2021-11-16
How to Cite
He, S., Hao, Z., Wang, D., Guo, Y., Wu, H., Ali, A., Zheng, R., Zheng, X., Chen, J., & Shi, J. (2021). Formamide deionized accelerates the somatic embryogenesis of Cunninghamia lanceolata. Forest Systems, 30(3), e016. https://doi.org/10.5424/fs/2021303-18068
Issue
Vol. 30 No. 3 (2021)
Section
Research Articles

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