The use of polyethylene glycol to reduce the anti-nutritional effects of tannins in Cistus ladanifer L.

  • Maria Teresa P. Dentinho Instituto Nacional de Investigação Agrária e Veterinária, Pólo Investigação da Fonte Boa (INIAV-Fonte Boa), 2005-048 Santarém Centro Investigação Interdisciplinar em Sanidade Animal (CIISA), Avda Universidade Técnica, 1300-477 Lisboa
  • Olga C. Moreira Instituto Nacional de Investigação Agrária e Veterinária, Pólo Investigação da Fonte Boa (INIAV-Fonte Boa), 2005-048 Santarém
  • Rui J. B. Bessa Centro Investigação Interdisciplinar em Sanidade Animal (CIISA), Avda Universidade Técnica, 1300-477 Lisboa Faculdade de Medicina Veterinária, Universidade Lisboa (ULisboa), Avda Universidade Técnica, 1300-477 Lisboa
Keywords: condensed tannins, rumen degradability, rumen fermentation, PEG, RUSITEC


Aim of study: To evaluate the impact of Cistus ladanifer L. (rockrose) tannins on ruminal degradability and fermentation characteristics and the use of polyethylene glycol (PEG), as feed additive, to mitigate the anti-nutritional effects of rockrose tannins.

Material and methods: Aerial parts of rockrose plants were harvested in March, freeze dried and divided in 4 subsamples which were treated with 0, 25, 50 and 75 g of PEG/kg of dry matter (DM). The mixtures were analysed for chemical composition including total phenolics, total tannins and condensed tannins. In situ rumen organic matter (OM) and N rumen degradability was evaluated using three rumen-cannulated rams and ruminal fermentation pattern (volatile fatty acids (VFA), gas production) was evaluated in vitro using a Rumen Simulation Technique (RUSITEC) apparatus.

Main results: In situ experiment indicated that the effective degradability of the OM and N increased linearly (p<0.05) with PEG inclusion due to an increase of the degradation rate (p<0.05). RUSITEC data indicated that substrate disappearance and gas and VFA production increased linearly (p<0.05) with PEG inclusion.

Research highlights: Inclusion of PEG to C. ladanifer feed was effective to prevent the anti-nutritive effects of tannins. Thus, the use of PEG as feed additive can promote a better utilization of this shrub by ruminants.


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How to Cite
Dentinho, M. T. P., Moreira, O. C., & Bessa, R. J. B. (2018). The use of polyethylene glycol to reduce the anti-nutritional effects of tannins in Cistus ladanifer L. Forest Systems, 27(1), e04S.