Mini-cutting technique for the propagation of a Eucalyptus camaldulensis clone selected in a semiarid region
Abstract
Aim of study: To assess the effectiveness of the mini-cutting technique in propagating a clone of Eucalyptus camaldulensis Dehnh selected in semiarid conditions, test the impact of the growth regulators Indole-3-Acetic Acid (IAA) and Indole-3-Butyric Acid (IBA) on rooting and determine the dynamics of adventitious rooting. At the same time, an anatomical study was conducted to examine the tissues involved in the formation of adventitious roots.
Area of study: Mossoró, located in the state of Rio Grande do Norte, Brazil.
Material and methods: The mini-stumps of the E. camaldulensis clone were subjected to five consecutive prunings and the shoots were analyzed for their adventitious rooting capacity by testing the IAA and IBA auxins at four doses (0 -control; 1,000; 2,000; and 4,000 mg.L-1). A temporal analysis of rooting was carried out with evaluations performed every 7 days from the day of staking until day 28 and samples were collected to peform the anatomic analysis.
Main results: The mini-stumps from E. camaldulensis clone showed 100% survival, 13 propagules per mini-stump/month and 900 sprouts/m2/month. The adventitious rooting was lower in control (55%) than in IAA (85%) and IBA (80%) at 2.000 mg.L-1. Rhizogenesis of the mini-cuttings started at day 14. The IAA and IBA increased mini-cutting rooting speed. Root formation was direct from phloem or vascular cambium in control and from xylem, phloem or vascular cambium in mini-cuttings treateds with IAA and IBA.
Research highlights: The E. camaldulensis clone showed strong potential for propagation under semiarid conditions, with rooting and survival rates above 80% when treated with auxins. These results confirm the mini-cutting technique as an efficient and practical solution for large-scale propagation, offering valuable applications in commercial forestry under challenging environmental conditions.
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