Relationships between diameter growth and functional wood anatomy in Eucalyptus globulus clones

Introduction

The resistance of xylem vessels to water movement depends primarily on their anatomical characteristics (vessel diameter - VD, and vessel number - VN, interconnection, and spatial arrangement). Larger (wider) vessels have lower resistance to water flow than narrow vessels, resulting in higher hydraulic conductivity (Ks), which is generally correlated with high stomatal conductance, carbon fixation, and growth in woody species (Santiago et al., 2004SantiagoLS, GoldsteinG, MeinzerFC, FisherJB, MachadoK, WoodruffD, et al., 2004. Leaf photosynthetic traits scale with hydraulic conductivity and wood density in Panamanian forest canopy trees. Oecologia140: 543-550. 10.1007/s00442-004-1624-1; Brodribb et al., 2005BrodribbTJ, HolbrookNM, HillRS, 2005. Seedling growth in conifers and angiosperms: impacts of contrasting xylem structure. Aust J Bot53: 749-755. 10.1071/BT05049). However, it is recognized that there is a trade-off between the maximum capacity to move water over long distances within the xylem and its vulnerability to cavitation (Tyree & Zimmermann, 2002TyreeMT, ZimmermannMH, 2002. Xylem structure and the ascent of sap, Springer, Berlin. 284 pp. 10.1007/978-3-662-04931-0; Hacke et al., 2006HackeUG, SperryJS, WheelerJK, CastroL, 2006. Scaling of angiosperm xylem structure with safety and efficiency. Tree Physiol26: 689-701. 10.1093/treephys/26.6.689). This implies a potential trade-off between growth and resistance to drought stress in plants. To adjust water requirements according to the growth environment, plants can alter wood properties in several ways, such as varying vessel diameter or number, varying the lumen fraction (F, the fraction of sapwood occupied by vessel lumens), varying vessel composition (S, a measure of vessel diameter distribution), and varying the total area of sapwood supplying water to a specific leaf area (Zanne et al., 2010ZanneAE, WestobyM, FalsterDS, AckerlyDA, LoarieSR, ArnoldSEJ, et al., 2010. Angiosperm wood structure: global patterns in vessel anatomy and their relation to wood density and potential conductivity. Am J Bot97: 207-215. 10.3732/ajb.0900178). These variations depend in part on the genotype (genotypic variation) and in part on the ability to change in response to fluctuations in environmental factors (phenotypic variation or plasticity) (e.g. Pausftch et al., 2016PfautschS, HarbuschM, WesolowskiA, SmithR, MacfarlaneC, TjoelkerMG, et al., 2016. Climate determines vascular traits in the ecologically diverse genus Eucalyptus. Ecol Lett19: 240-248. 10.1111/ele.12559).

The aforementioned relationships between functional wood anatomy, growth, and drought stress resistance have been extensively studied at the inter-specific level, exploring wide ranges of variation in wood traits (e.g., Wheeler et al., 2005WheelerJK, SperryJS, HackeUG, HoangN, 2005. Inter-vessel pitting and cavitation in woody Rosaceae and other vesselled plants: a basis for a safety versus efficiency trade-off in xylem transport. Plant Cell Environ28: 800-812. 10.1111/j.1365-3040.2005.01330.x; Hacke et al., 2006HackeUG, SperryJS, WheelerJK, CastroL, 2006. Scaling of angiosperm xylem structure with safety and efficiency. Tree Physiol26: 689-701. 10.1093/treephys/26.6.689, 2009HackeUG, JacobsenAL, PrattRB, 2009. Xylem function of arid land shrubs from California, USA: an ecological and evolutionary analysis. Plant Cell Environ32: 1324-1333. 10.1111/j.1365-3040.2009.02000.x; Gleason et al., 2016GleasonSM, WestobyM, JansenS, ChoatB, HackeUG, PrattRB, et al., 2016. Weak tradeoff between xylem safety and xylem-specific hydraulic efficiency across the world's woody plant species. New Phytol209: 123-136. 10.1111/nph.13646). However, relationships at the intra-species or intra-genus level are scarcer, and at these levels, examples can be found that bypass the mentioned trade-offs between efficiency and safety of the conducting system (e.g., Lobo et al., 2018LoboA, Torres-RuizJM, BurlettR, LemaireC, PariseC, FrancioniC, et al., 2018. Assessing inter- and intraspecific variability of xylem vulnerability to embolism in oaks. For Ecol Manage424: 53-61. 10.1016/j.foreco.2018.04.031 at the intra-specific level in Quercus petraea; Fernández et al., 2019FernándezME, BarottoAJ, Martínez MeierA, GyengeJE, TesónN, Quiñones MartorelloAS, et al., 2019. New insights into wood anatomy and function relationships: how Eucalyptus challenges what we already know. For Ecol Manage454: 117638. 10.1016/j.foreco.2019.117638 at the inter-specific level in Eucalyptus spp). Nevertheless, given the generally positive relationship between vessel size and maximum xylem hydraulic conductivity (Ks_max, measured independently of anatomy), it is expected that the relationship between Ks_max and growth will also be positive at the intra-specific (or intra-genus) level.

Considering the relationships between wood anatomy and tree growth at the inter-specific level, comparative analyses of several tropical species have reported positive correlations between vessel diameter or area and growth ‒in terms of stem diameter, total height, and growth ring width‒ (Fichter & Worbes, 2012FichterE, WorbesM,2012. Wood anatomical variables in tropical trees and their relation to site conditions and individual tree morphology. IAWA J33(2): 119-140. 10.1163/22941932-90000084; Hietz et al., 2017HietzP, RosnerS, Hietz-SeifertU, WrightSJ, 2017. Wood traits related to size and life history of trees in a Panamanian rainforest. New Phytol213: 170-180. 10.1111/nph.14123). Principal component analyses in these studies indicated that between 34-50% of the observed variation reflected the positive relationship between growth and variables associated with water transport efficiency (vessel diameter and area, vessel composition ‒ S ‒ and vulnerability index) and a negative relationship with vessel frequency (Fichter & Worbes, 2012FichterE, WorbesM,2012. Wood anatomical variables in tropical trees and their relation to site conditions and individual tree morphology. IAWA J33(2): 119-140. 10.1163/22941932-90000084; Hietz et al., 2017HietzP, RosnerS, Hietz-SeifertU, WrightSJ, 2017. Wood traits related to size and life history of trees in a Panamanian rainforest. New Phytol213: 170-180. 10.1111/nph.14123). Furthermore, when individual tree size is included in the models, hydraulic conductivity scales positively with maximum tree size (Hietz et al., 2017HietzP, RosnerS, Hietz-SeifertU, WrightSJ, 2017. Wood traits related to size and life history of trees in a Panamanian rainforest. New Phytol213: 170-180. 10.1111/nph.14123). In contrast, no relationship was discovered between vessel diameter and tree size (measured as stem diameter) in interspecific comparisons conducted within the genus Eucalyptus in both a common garden and an environmental gradient, but a positive relationship with climate (aridity index) was apparent. These studies concluded that the hydraulic anatomy of these species is primarily determined by climate and genetics, rather than tree size (Pausftch et al., 2016PfautschS, HarbuschM, WesolowskiA, SmithR, MacfarlaneC, TjoelkerMG, et al., 2016. Climate determines vascular traits in the ecologically diverse genus Eucalyptus. Ecol Lett19: 240-248. 10.1111/ele.12559; Bourne et al., 2017BourneAE, CreekD, PetersJMR, EllsworthDS, ChoatB, 2017. Species climate range influences hydraulic and stomatal traits in Eucalyptus species. Ann Bot120: 123-133. 10.1093/aob/mcx020; Santos et al., 2021SantosDRS, SantosRF, AnciottiJLR, Silva-NetoCM, SilvaAS, NovaesE, et al., 2021. Growth differential related to wood structure and function of Eucalyptus spp. clones adapted to seasonal drought stress. Forest Syst30(3): e014. 10.5424/fs/2021303-17908).

At the intra-specific level, some recent studies have shown variable relationships between anatomy and growth. For ring-porous species, positive correlations between tree ring width or stem diameter and hydraulic vessel diameter have been reported (Camarero et al., 2021CamareroJJ, ColangeloM, Rodríguez-GonzalezPM, Sanchez-MirandaA, Sanchez-SalgueroR, CampeloF, et al., 2021. Wood anatomy and tree growth covary in riparian ash forests along climatic and ecological gradients. Dendrochronologia70: 125891. 10.1016/j.dendro.2021.125891 in Fraxinus sp; Hietz et al., 2022HietzP, RungwattanaK, ScheffknechtS, GeorgeJP, 2022. Effects of provenance, growing site, and growth on Quercus robur wood anatomy and density in a 12-year-old provenance trial. Front Plant Sci13: 795941. 10.3389/fpls.2022.795941 in Quercus sp). These authors concluded that wood production and hydraulic conductivity are coordinated by the development of large vessels in earlywood, which allows for high growth rates. Tree size explained the highest percentage of the observed variation in vessel size, in contrast to the lesser influence of other factors studied in these species (site, provenance, and their interaction). Studies at the intra-specific level within the genus Eucalyptus, which presents diffuse-porous wood, show different behavior of xylem structural variables among commercial clones/hybrids in response to extreme environmental events (drought), resulting in different individual growth rates (Camara et al., 2020CamaraAP, VidaurreGB, OliveiraJCL, de Toledo PicoliJA, AlmeidaNMF, RoqueRM, et al., 2020. Changes in hydraulic architecture across a water availability gradient for two contrasting commercial Eucalyptus clones. For Ecol Manage474: 118380. 10.1016/j.foreco.2020.118380; Binkley et al., 2022BinkleyD, CampoOC, Alcarde AlvaresC, Lorenzato CarneiroR, StapeJL, 2022. Variation in whole-rotation yield among Eucalyptus genotypes in response to water and heat stresses: The TECHS Project. For Ecol Manage462: 117953. 10.1016/j.foreco.2020.117953; Chambi-Legoas et al., 2023Chambi-LegoasR, Tomazello-FilhoM, LaclauJP, ChaixeG, 2023. Potassium fertilization enhances xylem plasticity and growth recovery of Eucalyptus grandis trees in response to drastic changes in water availability. For Ecol Manage528: 120656. 10.1016/j.foreco.2022.120656). However, to our knowledge there are no publications quantifying these relationships at the intra-specific (and clone) level within the genus Eucalyptus.

The genus Eucalyptus comprises more than 700 species occupying a wide range of environmental conditions and developing a large variation in strategies for environmental stress tolerance (Pausftch et al., 2016PfautschS, HarbuschM, WesolowskiA, SmithR, MacfarlaneC, TjoelkerMG, et al., 2016. Climate determines vascular traits in the ecologically diverse genus Eucalyptus. Ecol Lett19: 240-248. 10.1111/ele.12559). Among the species in this genus, Eucalyptus globulus Labill. stands out for its high industrial wood quality and extensive global commercial use, which indeed is geographically limited by its sensitivity to frost and high temperatures, but exhibits moderate drought tolerance. For this species and others of the same genus cultivated in temperate and subtropical areas of South-America, differences in wood anatomy and density have been demonstrated in different sites, suggesting the adaptive value of xylem anatomical changes in this genus (Barotto et al., 2016BarottoAJ, FernándezME, GyengeJE, Martínez MeierA, MeyraA, MonteolivaS, 2016. First insights into the functional role of vasicentric tracheids and parenchyma in Eucalyptus species with solitary vessels: Do they contribute to xylem efficiency or safety?Tree Phy36: 1485-1497. 10.1093/treephys/tpw072, 2017; Moreno et al., 2018MorenoK, SpaventoEM, MonteolivaS, 2018. Variabilidad en la anatomía y densidad de la madera de Eucalyptus globulus en Argentina: efecto de la procedencia, suelo y edad. Rev Fac Agr La Plata117: 163-170.; Fernández et al., 2019FernándezME, BarottoAJ, Martínez MeierA, GyengeJE, TesónN, Quiñones MartorelloAS, et al., 2019. New insights into wood anatomy and function relationships: how Eucalyptus challenges what we already know. For Ecol Manage454: 117638. 10.1016/j.foreco.2019.117638). According to previous studies, despite showing high stem growth rates in temperate areas (25-35 m³ ha^-1 year^-1), E. globulus is characterized by an anatomy of relatively small and not very numerous vessels, resulting in low theoretical hydraulic conductivity (Ks) compared to other Eucalyptus species, such as E. grandis. However, although vessel size is important in determining Ks, it has been shown for several species within the genus that the total lumen area measured by the F index has a higher relative influence than vessel composition (measured by the parameter S) (Barotto et al., 2017BarottoAJ, MonteolivaS, GyengeJ, Martinez MeierA, MorenoK, TesónN, et al., 2017. Wood density and anatomy of three Eucalyptus species: implications on hydraulic conductivity. Forest Syst26: 1-11. 10.5424/fs/2017261-10446). Furthermore, F is more stable within and between species, while S shows higher variation, suggesting that it has a more plastic nature and/or is more susceptible to variations due to natural selection (genetic variation). An analysis of 3005 angiosperm species showed that F and S are not correlated with each other and can vary independently in response to different selection factors (Zanne et al., 2010ZanneAE, WestobyM, FalsterDS, AckerlyDA, LoarieSR, ArnoldSEJ, et al., 2010. Angiosperm wood structure: global patterns in vessel anatomy and their relation to wood density and potential conductivity. Am J Bot97: 207-215. 10.3732/ajb.0900178).

In this context, the objectives of this study were to: 1) analyze the variability of hydraulic anatomical parameters in clones of E. globulus with different growth rates, and 2) determine if the mean magnitude of growth (measured as two growth groups) affects the relationships between anatomical variables and individual mean growth in E. globulus clones.

We expected that the correlations between functional anatomical variables and individual growth (measured as diameter at breast height ‒ DBH ‒ at a common age) to have different signs and/or magnitudes between the growth groups (high and low growth clones), driven by groups´ differences in the genotype × environment interaction.

Due to the variable antecedents in the genus (none of them at the level studied here) is not possible to anticipate the particular sign of these relationships within each group. However, we expect that these relationships do differ as a result of different wood anatomical responses to common environmental signals by the different clones, which largely explain their different growth rates.

Material and methods

Sampling was conducted in a clone productivity trial of E. globulus established in 1996 by the Instituto Nacional de Tecnología Agropecuaria (INTA) of Argentina. This trial is located at the Experimental Station of INTA in Balcarce, Buenos Aires Province (37°46' S; 58°18' W; 160 meters above sea level). At the time of the wood sampling, the trees in the trial were 25 years old. The genetic material examined in this study consisted of 10 clones selected and released by the Portuguese company Soporcel SA, 5 of high growth (HG) and 5 of low growth (LG) under the environmental conditions of the study area (Table 1). This classification was made after analyzing the growth patterns of the trees in situ based on the remeasurement of DBH. Based on this analysis (unpublished data), 3 clone groups, corresponding to high, low, and intermediate growth were defined, with the two extremes selected for the present study. In this regard, clones with mean DBH greater than 30 cm were selected as HG and those with mean DBH lower than 21 cm were selected as LG. In this study, the DBH at 25 years was taken as a proxy for mean growth of the clones since the plantation.

A core sample was taken from the stem of each selected tree at DBH with a 5.15 mm in diameter Pressler increment borer, from pith to bark, for further wood anatomical determinations.

Wood anatomical parameters were estimated from serial transverse histologic sections taken at three radial positions proportional to the radius length in each wood core. These positions corresponded to inner wood (closest to the pith, between 70-55% of the radius length), middle wood (between 70-85% of the radius length), and outer wood (adjacent to the bark, between 100-85% of the radius length). The sections were stained with 1% toluidine blue, observed under a light microscope (Olympus CX31, Japan), and digital images were captured at 4x magnification for vessel measurement. The following measurements were taken for all vessels using Image Pro software (v 6.0, Media Cybernetics, USA): vessel diameter (VD, μm) and number of vessels per unit area (VN, n·mm^-2). After that, we estimated the fraction of lumens (F) = VA·VN (VA = vessel area), the vessel composition (S) = VA/VN, and the theoretical specific hydraulic conductivity (Ks, kg·m^-1·MPa^-1·s^-1).

The hydraulic conductivity kh was calculated using the Hagen-Poiseuille formula (Tyree & Ewers, 1991TyreeMT, EwersFW, 1991. The hydraulic architecture of trees and others woody plants. New Phytol119: 345-360. 10.1111/j.1469-8137.1991.tb00035.x):

where kh is the xylem hydraulic conductivity, ρ is the density of water (kg·m^-³), d is the diameter of the vessel lumen (m), and ηw is the viscosity of water (MPa·s). Ks is the specific hydraulic conductivity and it was estimated as Ks = kh / xylem area where vessels were measured.

Results

Variability between growth groups

 ⌅

Except for the variables F and Ks, significant differences (p<0.05) were observed between the HG and LG groups of E. globulus (Table 2). The DBH showed, as expected, a significantly higher value in HG than in LG trees, with the former trees having almost twice the DBH of those in the LG group (Table 2). The parameters that showed the highest variability (i.e. higher coefficient of variation, Table 2) were VN, F, S, and Ks, with higher variability in trees from the LG group than the HG group. VD showed similar variation in both groups (Table 2).

Table 2 Diameter at breast height (DBH), vessel functional anatomy (VD: vessel diameter, VN: number of vessels per xylem unit area, F: lumen fraction, S: vessel composition), and theoretical specific hydraulic conductivity (Ks) of 10 clones of Eucalyptus globulus grouped by growth rate: high growth (HG) and low growth (LG). Mean and coefficient of variation (%). 

	DBH (cm)	VD (µm)	VN (nº·mm-2)	F	S (mm4)	Ks (kg·m-1·MPa-1·s-1)
HG, n=18	31.79b 14.22%	87.87b 12.61%	7.57a 15.91%	0.0458a 22.40%	0.00082b 30.68%	22.47a 41.50%
LG, n=23	17.92a 25.78%	77.38a 13.88%	9.93b 29.02%	0.0469a 34.66%	0.00052a 39.50%	18.94a 55.23%

[i] 

n = number of sampled trees. Different letters indicate significant differences between HG and LG groups (Kruskal-Wallis non-parametric test, p<0.05).

The studied E. globulus clones showed significant differences (p<0.05) in VD and VN, with HG trees having larger vessel diameter and fewer vessels per unit xylem area compared to trees in the LG group. In addition, HG trees showed a greater variation in S (Table 2 & Fig. 1). It was also observed that the most frequent values of vessel diameter in HG trees were around 105 µm, while in LG trees it ranged from 75 to 105 µm (Fig. 1). Nevertheless, these differences in vessel size and number were not reflected in the theoretical Ks, which did not differ between HG and LG trees (p>0.05).

  

Figure 1 Frequency distribution (proportion of vessels of each size category in relation to all vessels) of vessel diameter (VD) for E. globulus categorized by diameter growth. HG = high mean growth; LG = low mean growth. 

Variability among clones within each growth group

 ⌅

Clones within each growth group showed different behavior for some anatomical variables (Table 2, Fig. 2). Regarding VD, only 3 clones within the HG group produced large vessels (clones 516, 513, 504), while the other 2 clones (508 and 503) formed vessels with significantly (p<0.05) smaller diameters (similar to the vessels of the LG clones, see Table S1 [suppl]). HG clones 516, 513 and 504 also showed the highest F values and the other 2 clones (508 and 503) the lowest. In the LG group, only clone 520 presented low F values, the remaining 4 clones were like the HG group. However, for VN and S, the behavior of the clones within each growth group was more homogeneous (Fig. 2, i.e. there were no significant differences between clones within the groups according to the non-parametric analysis, p>0.05, Table S1 [suppl]). In this regard, all clones in the HG group produced wood with fewer vessels per unit area and a wider diameter distribution (high S value) compared to clones in the LG group. Finally, Ks, as an integrative variable of the other parameters, showed a high variability among clones, with non-significant differences (p>0.05) between the HG and LG groups (Fig. 3, Table S1 [suppl]). Only two genotypes from the HG group showed significant differences with another genotype from the LG group (clones 504 and 513 vs. clone 512).

  

Figure 2 Variability in hydraulic anatomy between high-growth (HG: to the left of the dotted vertical line) and low-growth (LG: to the right of the dotted vertical line) clones (mean and confidence intervals, DBH values plotted in gray). VD: vessels diameter. VN: number of vessels per xylem unit area. F: fraction of lumens. S: vessels composition. 

  

Figure 3 Variability in theoretical specific hydraulic conductivity (Ks) among high-growth (HG) and low-growth (LG) clones (mean and confidence intervals, DBH values plotted in gray). 

Discussion

The study's findings, which compared the HG and LG clone groups and examined genetically selected E. globulus clones planted in a common garden trial in the Argentine Pampa region, revealed differences in the degree of variability and the relationships between these variables and individual tree growth (measured here by the DBH of the trees at a common age). In fact, the moderate relationships observed within the LG group disappeared within the HG group, but reappeared when all trees were included in the analysis, suggesting that the large variation in values across all clones and individuals studied was responsible for the observed patterns.

The variability among clones within each growth group was high for some variables (VD, F) and low for others (S, Ks) (Figs. 2 and 3). Some authors have postulated that the low variability found in Eucalyptus hydraulic anatomy is due to a strong genetic limitation (Bourne et al., 2017BourneAE, CreekD, PetersJMR, EllsworthDS, ChoatB, 2017. Species climate range influences hydraulic and stomatal traits in Eucalyptus species. Ann Bot120: 123-133. 10.1093/aob/mcx020; Santos et al., 2021SantosDRS, SantosRF, AnciottiJLR, Silva-NetoCM, SilvaAS, NovaesE, et al., 2021. Growth differential related to wood structure and function of Eucalyptus spp. clones adapted to seasonal drought stress. Forest Syst30(3): e014. 10.5424/fs/2021303-17908). For instance, in an inter-specific study of 6 Eucalyptus species with different climatic adaptations (rainfall ranges), Bourne et al. (2017BourneAE, CreekD, PetersJMR, EllsworthDS, ChoatB, 2017. Species climate range influences hydraulic and stomatal traits in Eucalyptus species. Ann Bot120: 123-133. 10.1093/aob/mcx020) showed that genetics still limits phenotypic plasticity in hydraulic anatomical traits when growing in the same environment (common garden). They also showed that other rapidly responsive physiological variables (water relations, stomatal closure) were positively related to growth, suggesting that different water use strategies among different genotypes (species in their study) led to higher productivity and growth. Similarly, Santos et al. (2021SantosDRS, SantosRF, AnciottiJLR, Silva-NetoCM, SilvaAS, NovaesE, et al., 2021. Growth differential related to wood structure and function of Eucalyptus spp. clones adapted to seasonal drought stress. Forest Syst30(3): e014. 10.5424/fs/2021303-17908) found differences in hydraulic anatomy and growth in 4 interspecific hybrid clones of Eucalyptus growing in a common garden in Brazil. These authors indicated that trends in the relationships between anatomical parameters and growth depended on the clone evaluated. For example, vessel composition (S) was positively related to volume growth, meanwhile VD was related to DBH in some genotypes. These authors also concluded that high Ks did not necessarily imply higher growth rates. These results are consistent with what was observed in our study on E. globulus, although we cannot demonstrate genetic limitations of the selected clones.

In the present study, the relationships between anatomical-functional variables related to hydraulic efficiency and the DBH showed partially different behavior depending on the growth group. HG clones did not show significant correlations between hydraulic variables and DBH. In contrast, in the LG group, significant associations were found between two of the variables analyzed (VN and S) and diameter growth. Therefore, partial evidence was found in relation to our expectations, suggesting that the potential genetic differences among the clones and their interactions with the environment could influence this differential behavior within growth groups (e.g. Santos et al., 2021SantosDRS, SantosRF, AnciottiJLR, Silva-NetoCM, SilvaAS, NovaesE, et al., 2021. Growth differential related to wood structure and function of Eucalyptus spp. clones adapted to seasonal drought stress. Forest Syst30(3): e014. 10.5424/fs/2021303-17908). The lack of significant relationships within the HG group suggests that the different clones achieved high growth through different hydraulic strategies. Unfortunately, due to the low number of replicates per clone, it was not possible to evaluate correlations within clones to elucidate alternative relationships between clones. On the other hand, when all trees were analyzed, regardless of the growth group, some significant trends were observed, including the relationship between the amplitude of xylem vessel size, measured here by the S parameter, and DBH. At the same time, our results suggest that xylem hydraulic efficiency, estimated as theoretical Ks, does not have a significant effect on the DBH in E. globulus. This behavior is likely due to opposite changes in vessel size and number (Table 2) resulting in a similar area occupied by lumens (F), which has a greater impact on Ks than S (Barotto et al., 2017BarottoAJ, MonteolivaS, GyengeJ, Martinez MeierA, MorenoK, TesónN, et al., 2017. Wood density and anatomy of three Eucalyptus species: implications on hydraulic conductivity. Forest Syst26: 1-11. 10.5424/fs/2017261-10446).

Vessel size amplitude (S) in branches has been correlated in Eucalyptus spp with the safety of the conductive system (larger diameter amplitude, lower vulnerability to cavitation) both at the inter-specific level (Barotto et al., 2016BarottoAJ, FernándezME, GyengeJE, Martínez MeierA, MeyraA, MonteolivaS, 2016. First insights into the functional role of vasicentric tracheids and parenchyma in Eucalyptus species with solitary vessels: Do they contribute to xylem efficiency or safety?Tree Phy36: 1485-1497. 10.1093/treephys/tpw072; Fernández et al., 2019FernándezME, BarottoAJ, Martínez MeierA, GyengeJE, TesónN, Quiñones MartorelloAS, et al., 2019. New insights into wood anatomy and function relationships: how Eucalyptus challenges what we already know. For Ecol Manage454: 117638. 10.1016/j.foreco.2019.117638) and within E. globulus (Fernández et al., 2019FernándezME, BarottoAJ, Martínez MeierA, GyengeJE, TesónN, Quiñones MartorelloAS, et al., 2019. New insights into wood anatomy and function relationships: how Eucalyptus challenges what we already know. For Ecol Manage454: 117638. 10.1016/j.foreco.2019.117638). In these studies, this variable was also associated with a high maximum Ks (measured hydraulically). In the present study, clones with high S, which also had the highest DBH, had theoretical Ks that were either high or low, decoupling the S-Ks association seen in the previously stated studies. In this regard, it appears that the amplitude of vessel diameter is more important in relation to growth than hydraulic efficiency itself. A possible explanation for this result could be that the growth was measured under field conditions, not as potential growth without resource and climatic constraint.

Conclusions

Studies of functional anatomy and its relationship to growth in commercial Eucalyptus clones are very recent, so it is important to expand the knowledge in these areas for use in genetic improvement programs. The different genotypes characterized by differential growth rates appear to have different hydraulic strategies. These different strategies are reflected in the differential variability in anatomical-functional variables observed between the HG and LG groups and among different clones. However, contrary to what has been postulated in various woody species, there is generally no clear relationship between theoretical xylem hydraulic efficiency (Ks) and growth (measured here as DBH) in the genotypes studied. Instead, a relationship was found between vessel size amplitude (S) and DBH. Consequently, studies are needed to elucidate the relationship between Ks and growth under non-limited (potential) growth conditions, as well as the impact of vascular system safety (vulnerability to cavitation) on maximum potential growth and under field conditions. This will contribute to the identification of key genetic selection traits for different environmental conditions in economically important forest species.